1/1/2023 0 Comments Catlight jenkinsThe basal ganglia are important for a wide range of behaviors from decision making to movement ( Graybiel, 2005). The choice of tdTomato as a reporter may also prove useful in other circuits in which the simultaneous visualization of two defined cell populations is desired. In combination with the existing GENSAT BAC transgenic line identifying the indirect pathway ( Drd2-EGFP), this new transgenic line creates a novel platform for the study of basal ganglia physiology. Using the regulatory promoter elements of the type 1a dopamine receptor gene ( Drd1a), we created a BAC transgenic line that identifies the direct pathway of the basal ganglia ( Drd1a-tdTomato). Historically, the creation of transgenic lines expressing non-EGFP fluorophores in defined neuronal populations has been met with only limited success, possibly because of toxicity issues, particularly for red spectral variants ( Vintersten et al., 2004 Long et al., 2005). Here we report the successful application of tdTomato protein for this purpose. As a result, the ability to simultaneously visualize two cell populations in the same preparation is limited by the lack of lines using contrasting fluorophores. One limitation of this technology is that the majority of BAC transgenic mice harnessing this technology use enhanced green fluorescent protein (EGFP) as the fluorophore. The expression of fluorescent proteins in defined subsets of neurons has become a powerful tool for neurobiology.
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